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magnetic cell separation kits  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec magnetic cell separation kits
    Magnetic Cell Separation Kits, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dendritic+cells/Myeloid+Dendritic+Cell+Isolation+Kit%2C+human/pm42520804-271-13-17
    Average 94 stars, based on 37 article reviews
    magnetic cell separation kits - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Isolation:

    Article Title: Vaginal Prevotella timonensis Bacteria Enhance HIV‐1 Uptake and Differentially Affect Transmission by Distinct Primary Dendritic Cell Subsets
    Article Snippet: .. The human CD1c + isolation kit for dendritic cells (Miltenyi Biotec) was used for the isolation of CD1c + DCs from peripheral blood mononuclear cells (PBMCs) by magnetic labelling and separation according to the manufacturer's instructions. ..

    Article Title: Vaginal Prevotella timonensis Bacteria Enhance HIV-1 Uptake and Differentially Affect Transmission by Distinct Primary Dendritic Cell Subsets.
    Article Snippet: .. The human CD1c+ isolation kit for dendritic cells (Miltenyi Biotec) was used for the isolation of CD1c+ DCs from peripheral blood mononuclear cells (PBMCs) by magnetic labelling and separation according to the manufacturer’s instructions. ..

    other:

    Article Title: GSK3β Inhibition by Phosphorylation at Ser 389 Controls Neuroinflammation
    Article Snippet: CD11b-PEVio770 , REA592 , Macrophages, microglia, granulocytes, NK cells, and subsets of dendritic cells , 1:50 , Miltenyi Biotec (130-113-246).

    Article Title: ESPEC-SUIT: a versatile and robust platform to identify and track antigen-specific T cell receptors in patients with cancer.
    Article Snippet: TCRαβ pairs of candidate clonotypes were extracted from single- cell V(D)J- sequencing results of post- ESPEC T cells or TIL using the makeTCR online tool (https:// edgreen21.shinyapps.io/makeTCR/).31 Details regarding the cloning procedure and constructs are described by Hamberger et al31 and at Addgene: makeTCR collection.

    Suspension:

    Article Title: Production of dendritic cell vaccines using different methods with equivalent results: Implications for emerging centers
    Article Snippet: Cell suspensions collected by apheresis underwent monocyte separation (CD14+ cells) using three different methods: i) Automated: automatic separation using a tubular system with magnetic beads (commercial system CliniMacs®); ii) Manual 1: monocyte purification by centrifugation gradients, using the Ficoll® and Percoll® reagents (Sigma Aldrich, USA), achieved by a single passage of the complete amount of leukapheresis material, and; iii) Manual 2: monocyte purification by centrifugation gradients, using the same procedure used for ‘Manual 1′, however divided into two consecutive passages, each with half of the leukapheresis material, to maintain the cell viability. .. The resulting cell suspension, regardless of the separation process, was adjusted to the concentration of 2 × 10 6 viable cells/ml in the specific culture medium for differentiation of dendritic cells (DendriMACSTM GMP Medium, Miltenyi Biotec, Germany) and subsequently plated in 150 mm treated culture dishes (Corning, NY, USA). .. For differentiation into immature dendritic cells, adherent cells were cultured at 37 °C in 5% CO2 for five days in DendriMACS Medium containing 100 IU/ml penicillin and streptomycin (10 μg/ml), 50 ng/ml MACS GMP recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) and 50 ng/ml MACS GMP recombinant human interleukin-4 (IL-4) (Miltenyi Biotec, Germany).

    Concentration Assay:

    Article Title: Production of dendritic cell vaccines using different methods with equivalent results: Implications for emerging centers
    Article Snippet: Cell suspensions collected by apheresis underwent monocyte separation (CD14+ cells) using three different methods: i) Automated: automatic separation using a tubular system with magnetic beads (commercial system CliniMacs®); ii) Manual 1: monocyte purification by centrifugation gradients, using the Ficoll® and Percoll® reagents (Sigma Aldrich, USA), achieved by a single passage of the complete amount of leukapheresis material, and; iii) Manual 2: monocyte purification by centrifugation gradients, using the same procedure used for ‘Manual 1′, however divided into two consecutive passages, each with half of the leukapheresis material, to maintain the cell viability. .. The resulting cell suspension, regardless of the separation process, was adjusted to the concentration of 2 × 10 6 viable cells/ml in the specific culture medium for differentiation of dendritic cells (DendriMACSTM GMP Medium, Miltenyi Biotec, Germany) and subsequently plated in 150 mm treated culture dishes (Corning, NY, USA). .. For differentiation into immature dendritic cells, adherent cells were cultured at 37 °C in 5% CO2 for five days in DendriMACS Medium containing 100 IU/ml penicillin and streptomycin (10 μg/ml), 50 ng/ml MACS GMP recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) and 50 ng/ml MACS GMP recombinant human interleukin-4 (IL-4) (Miltenyi Biotec, Germany).



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    Image Search Results


    CD34-derived cDC1s and cDC2s can be generated from blood progenitors. CD34-positive cells isolated from blood were differentiated into cDC1s and cDC2s in a 17-day protocol in which progenitors initially expand and afterward undergo skewing towards cDC1 and cDC2 differentiation. (A) Schematic representation of the 17-day protocol for DC generation. (B) Bar graph showing the percentage of cDC1s and cDC2s ± SEM at the end of the culture on day 17 ( n = 10). (C) Representative dot plot showing the presence of cDC1s and cDC2s based on the expression of CD141/CLEC9A and CD1c, respectively, after gating on viable single cells. (D) Violin plot showing the absolute number of cDC1s and cDC2s obtained with the current protocol, depending on the initial amount of cultured CD34-positive cells. Each dot represents the value of expanded cDC1s and cDC2s obtained from one independent donor ( n = 12). (E) Bar graphs show the percentage of positive DCs ± SEM for the given marker ( n = 3). CD34-derived cDC1s and cDC2s were characterized by flow cytometry to determine their lineage marker expression.

    Journal: Oncoimmunology

    Article Title: Ex vivo-generated conventional dendritic cells type 1 and type 2 from blood progenitors induce potent antigen-specific T-cell immunity

    doi: 10.1080/2162402X.2026.2695692

    Figure Lengend Snippet: CD34-derived cDC1s and cDC2s can be generated from blood progenitors. CD34-positive cells isolated from blood were differentiated into cDC1s and cDC2s in a 17-day protocol in which progenitors initially expand and afterward undergo skewing towards cDC1 and cDC2 differentiation. (A) Schematic representation of the 17-day protocol for DC generation. (B) Bar graph showing the percentage of cDC1s and cDC2s ± SEM at the end of the culture on day 17 ( n = 10). (C) Representative dot plot showing the presence of cDC1s and cDC2s based on the expression of CD141/CLEC9A and CD1c, respectively, after gating on viable single cells. (D) Violin plot showing the absolute number of cDC1s and cDC2s obtained with the current protocol, depending on the initial amount of cultured CD34-positive cells. Each dot represents the value of expanded cDC1s and cDC2s obtained from one independent donor ( n = 12). (E) Bar graphs show the percentage of positive DCs ± SEM for the given marker ( n = 3). CD34-derived cDC1s and cDC2s were characterized by flow cytometry to determine their lineage marker expression.

    Article Snippet: Alternatively, DCs were enriched by MACS using CD141-Microbeads (130-090-512, Miltenyi Biotec) and CD1c Isolation kit (130-119-475, Miltenyi Biotec) sequentially (see supplementary figure 1C).

    Techniques: Derivative Assay, Generated, Isolation, Expressing, Cell Culture, Marker, Flow Cytometry